Onetazid Mechanism of Action

Pharmacotherapeutic group: Anti-bacterials for systemic use. Third-generation cephalosporins.
Pharmacology: Pharmacodynamics: Mechanism of action: Ceftazidime inhibits bacterial cell wall synthesis following attachment to penicillin binding proteins (PBPs). This results in the interruption of cell wall (peptidoglycan) biosynthesis, which leads to bacterial cell lysis and death.
PK/PD relationship: For cephalosporins, the most important pharmacokinetic-pharmacodynamic index correlating with in vivo efficacy has been shown to be the percentage of the dosing interval that the unbound concentration remains above the minimum inhibitory concentration (MIC) of Ceftazidime for individual target species (i.e. %T>MIC).
Mechanism of Resistance: Bacterial resistance to Ceftazidime may be due to one or more of the following mechanisms: Hydrolysis by beta-lactamases. Ceftazidime may be efficiently hydrolysed by extended-spectrum beta-lactamases (ESBLs), including the SHV family of ESBLs and AmpC enzymes that may be induced or stably derepressed in certain aerobic Gram-negative bacterial species.
Reduced affinity of penicillin-binding proteins for Ceftazidime.
Outer membrane impermeability, which restricts access of Ceftazidime to penicillin binding proteins in Gram-negative organisms.
Bacterial efflux pumps.
Pharmacokinetics: Absorption: After intramuscular administration of 500 mg and 1 g of ceftazidime, peak plasma levels of 18 and 37 mg/L respectively are achieved rapidly. Five minutes after intravenous bolus injection of 500 mg, 1 g or 2 g, plasma levels are 46, 87 and 170 mg/L, respectively. The kinetics of ceftazidime are linear within the single dose range of 0.5 to 2 g following intravenous or intramuscular dosing.
Distribution: The serum protein binding of Ceftazidime is low at about 10%. Concentrations in excess of the MIC for common pathogens can be achieved in tissues such as bone, heart, bile, sputum, aqueous humour, synovial, pleural and peritoneal fluids. Ceftazidime crosses the placenta readily, and is excreted in the breast milk. Penetration of the intact blood-brain barrier is poor, resulting in low levels of Ceftazidime in the CSF in the absence of inflammation. However, concentrations of 4 to 20 mg/L or more are achieved in the CSF when the meninges are inflamed.
Biotransformation: Ceftazidime is not metabolised.
Elimination: After parenteral administration plasma levels decrease with a half-life of about 2 hrs. Ceftazidime is excreted unchanged into the urine by glomerular filtration; approximately 80 to 90% of the dose is recovered in the urine within 24 hrs. Less than 1% is excreted via the bile.