Berodual F UDV Mechanism of Action

Pharmacotherapeutic group: Adrenergics in combination with anticholinergics for obstructive airway diseases. ATC code: R03AL01.
Pharmacology: Mode of Action: Fenoterol hydrobromide/ipratropium bromide (Berodual F UDV) contains two active bronchodilating ingredients: ipratropium bromide, exhibiting an anticholinergic effect and fenoterol hydrobromide a beta-adrenergic agent.
Ipratropium bromide is a quaternary ammonium compound with anticholinergic (parasympatholytic) properties. In non-clinical studies, it inhibits vagally mediated reflexes by antagonising the action of acetylcholine, the transmitter agent released from the vagus nerve. Anticholinergics prevent the increase in intracellular concentration of Ca++ which is caused by interaction of acetylcholine with the muscarinic receptor on bronchial smooth muscle. Ca++ release is mediated by the second messenger system consisting of IP3 (inositol triphosphate) and DAG (diacylglycerol).
The bronchodilatation following inhalation of ipratropium bromide is primarily a local, site-specific effect, not a systemic one.
Non-clinical and clinical evidence suggest no deleterious effect of ipratropium bromide on airway mucous secretion, mucociliary clearance or gas exchange.
Fenoterol hydrobromide is a direct acting sympathomimetic agent, selectively stimulating beta₂-receptors in the therapeutic dose range. The stimulation of beta₁-receptors comes into effect at a higher dose range. Occupation of beta₂-receptors activates adenyl cyclase via a stimulatory G_s-protein.
The increase in cyclic AMP activates protein kinase A which then phosphorylates target proteins in smooth muscle cells. This in turn leads to the phosphorylation of myosin light chain kinase, inhibition of phosphoinositide hydrolysis, and the opening of large-conductance calcium-activated potassium channels.
Fenoterol hydrobromide relaxes bronchial and vascular smooth muscle and protects against bronchoconstricting stimuli such as histamine, methacholine, cold air, and allergen (early response). After acute administration the release of bronchoconstricting and pro-inflammatory mediators from mast cells is inhibited. Further, an increase in mucociliary clearance has been demonstrated after administration of doses of fenoterol (0.6 mg).
Higher plasma concentrations, which are more frequently achieved with oral, or even more so, with intravenous administration inhibit uterine motility. Also at higher doses, metabolic effects are observed: Lipolysis, glycogenolysis, hyperglycaemia and hypokalaemia, the latter caused by increased K+-uptake primarily into skeletal muscle. Beta-adrenergic effects on the heart such as increase in heart rate and contractility are caused by the vascular effects of fenoterol, cardiac beta₂-receptor stimulation, and at supratherapeutic doses, by beta₁-receptor stimulation. As with other beta-adrenergic agents, QTc prolongations have been reported. For fenoterol pressurised inhalation, solutions these were discrete and observed at doses higher than recommended. However, systemic exposure after administration with nebulisers (nebuliser solution, nebuliser solution in unit dose vials) might be higher than with recommended pressurised inhalation, solution doses. The clinical significance has not been established. Tremor is a more frequently observed effect of beta-agonists. Unlike the effects on the bronchial smooth muscle, the systemic effects on skeletal muscle of β-agonists are subject to the development of tolerance.
Concurrent use of these two active ingredients dilates the bronchi by affecting different pharmacological sites of action. The two active substances thus complement each other in their spasmolytic action on the bronchial muscles and allow a broad therapeutic use in the field of bronchopulmonary disorders associated with constriction of the respiratory tract. The complementary action is such that only a very low proportion of the β-adrenergic component is needed to obtain the desired effect, facilitating individual dosage suited to each patient with a minimum of adverse reactions.
Clinical Trials: Clinical efficacy and safety: In patients with asthma and COPD, better efficacy compared to its components ipratropium or fenoterol was demonstrated. Two studies (one with asthma patients, one with COPD patients) have shown that fenoterol hydrobromide/ipratropium bromide (Berodual F UDV) is as efficacious as double the dose of fenoterol administered without ipratropium but was better tolerated in cumulative dose response studies.
In acute bronchoconstriction, fenoterol hydrobromide/ipratropium bromide (Berodual F UDV) is effective shortly after administration and is therefore also suitable for treating acute episodes of bronchospasm.
Pharmacokinetics: The therapeutic effect of the combination ipratropium bromide and fenoterol hydrobromide is produced by a local action in the airway. The pharmacodynamics of the bronchodilation are therefore not related to the pharmacokinetics of the active constituents of the preparation.
Following inhalation 10 to 30% of a dose is generally deposited in lungs, depending on the formulation, inhalation technique and device, while the remainder of the delivered dose is deposited in the mouthpiece, mouth and the upper part of the respiratory tract (oropharynx). The portion of the dose deposited in the lungs reaches the circulation rapidly (within minutes). The amount of the active substance deposited in the oropharynx is slowly swallowed and passes the gastrointestinal tract. Therefore, the systemic exposure is a function of both oral and lung bioavailability.
There is no evidence that the pharmacokinetics of both ingredients in the combination differ from those of the mono-substances.
Fenoterol hydrobromide: Absorption: The absolute bioavailability following oral administration is low (approx. 1.5%).
The absolute bioavailability of fenoterol following inhalation is 18.7%. Absorption from the lung follows a biphasic course. 30% of the fenoterol hydrobromide is rapidly absorbed with a half-life of 11 minutes, and 70% is slowly absorbed with a half-life of 120 minutes.
Distribution: Fenoterol distributes widely throughout the body. About 40 % of the drug are bound to plasma proteins. In this 3-compartment model the apparent volume of distribution of fenoterol at steady state (Vdss) is approximately 189 L (≈ 2.7 L/kg).
Non-clinical studies with rats revealed that fenoterol and its metabolites do not cross the blood-brain barrier.
Biotransformation: Fenoterol undergoes extensive metabolism by conjugation to glucuronides and sulphates in humans. Following oral administration, fenoterol is metabolised predominantly by sulphation. This metabolic inactivation of the parent compound starts already in the intestinal wall.
Elimination: After inhalation via fenoterol hydrobromide + ipratropium bromide (Berodual) pressurised inhalation solution approximately 1% of an inhaled dose is excreted as free fenoterol in the 24-hour urine. Based on these data, the total systemic bioavailability of inhaled doses of fenoterol hydrobromide is estimated at 7%. Fenoterol has a total clearance of 1.8 L/min and a renal clearance of 0.27 L/min.
Kinetic parameters describing the disposition of fenoterol were calculated from plasma concentrations after i.v. administration. Following intravenous administration, plasma concentration-time profiles can be described by a 3-compartment model, whereby the terminal half-life is approximately 3 hours.
Following oral administration, total radioactivity excreted in urine was approximately 39% of dose and total radioactivity excreted in faeces was 40.2% of dose within 48 hours.
Ipratropium bromide: Absorption: Cumulative renal excretion (0-24 hrs) of ipratropium (parent compound) is below 1% of an oral dose and approximately 3 to 13% of an inhaled dose via Fenoterol hydrobromide + ipratropium bromide (Berodual) pressurised inhalation solution. Based on these data, the total systemic bioavailability of oral and inhaled doses of ipratropium bromide is estimated at 2% and 7 to 28% respectively. Taking this into account, swallowed dose portions of ipratropium bromide do not relevantly contribute to systemic exposure.
Distribution: Kinetic parameters describing the disposition of ipratropium were calculated from plasma concentrations after i.v. administration. A rapid biphasic decline in plasma concentrations is observed. The apparent volume of distribution at steady-state (Vdss) is approximately 176 L (≈ 2.4 L/kg). The drug is minimally (less than 20%) bound to plasma proteins. Non-clinical studies with rats and dogs, revealed that the quarternary amine ipratropium does not cross the blood-brain barrier. Binding of the main urinary metabolites to the muscarinic receptor is negligible and the metabolites have to be regarded as ineffective.
Biotransformation: After intravenous administration approximately 60% of a dose is metabolised, the major portion probably in the liver by oxidation.
Elimination: The half-life of the terminal elimination phase is approximately 1.6 hours. Ipratropium has a total clearance of 2.3 L/min and a renal clearance of 0.9 L/min.
In an excretion balance study cumulative renal excretion (6 days) of drug-related radioactivity (including parent compound and all metabolites) accounted for 9.3% after oral administration and 3.2% after inhalation. Total radioactivity excreted via the faeces was 88.5% following oral dosing and 69.4% after inhalation.
Toxicology: Single-dose toxicity: Single-dose toxicity studies with the combination ipratropium bromide and fenoterol hydrobromide in a ratio of 1/2.5 (ipratropium bromide/fenoterol hydrobromide) in mice and rats after oral, intravenous and inhalation administration revealed a low level of acute toxicity. In comparison to the individual components, the LD₅₀ values of the combination were determined more by the ipratropium bromide component than by fenoterol hydrobromide without any indication of potentiation.
Repeat-dose toxicity: Repeat-dose toxicity studies with the combination ipratropium bromide and fenoterol hydrobromide were performed in rats (oral, inhalation) and dogs (intravenous, inhalation) for up to 13 weeks. Only minor toxic effects at concentrations up to several hundred times greater than that recommended in man were observed. Left ventricular myocardial scars were seen only in one animal from the highest treatment group (84 mcg/kg/day) of the 4-week intravenous study in dogs. The 13-week oral study in rats and the 13-week inhalation study in dogs did not show any toxicological changes beyond that proportional to the individual components. There was no indication of potentiation with the combination in comparison to the individual components. All of the adverse effects observed are well known for fenoterol hydrobromide and ipratropium bromide.
Reproduction toxicity: After inhalation administration of the combination ipratropium bromide and fenoterol hydrobromide in rats and rabbits no teratogenic effects occurred. Also, no teratogenic effects were seen after ipratropium bromide, and after inhalation administration of fenoterol hydrobromide. After oral dosing, at doses >25 mg/kg/day (rabbits) and >38.5 mg/kg/day (mice) fenoterol hydrobromide induced an increase rate of malformations.
The malformations observed are considered a class effect for beta-agonists. Fertility was not impaired in rats at oral doses up to 90 mg/kg/day ipratropium bromide and up to 40 mg/kg/day fenoterol hydrobromide.
Genotoxicity: Genotoxicity studies for the combination were not performed. In vitro and in vivo assays revealed that neither fenoterol hydrobromide nor ipratropium bromide have a mutagenic potential.
Carcinogenicity: Carcinogenicity studies for the combination were not performed. No tumorigenic or carcinogenic effects were demonstrated in long term studies in dogs and rats or in carcinogenicity studies in mice and rats with ipratropium bromide. For fenoterol hydrobromide, carcinogenicity studies were performed after oral (mouse, 18 months rat, 24 months) and inhalation administration (rat, 24 months). At oral doses of 25 mg/kg/day an increased incidence of uterine leiomyomas with variable mitotic activity in mice and mesovarial leiomyomas in rats were observed. These findings are recognised effects caused by the local action of beta-adrenergic agents on the uterine smooth muscle cell in mice and rats. Taking into account the present level of research, these results are not applicable to man. All other neoplasias found were considered to be common types of neoplasia spontaneously occurring in the strains used and did not show a biologically relevant increased incidence resulting from treatment with fenoterol hydrobromide.